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| 16. |  | BEZERRA NETO, E.; BARRETO, L. P.; AZEVEDO NETO, A. D. de; SANTOS, V. Efeito da salinidade sobre dois cultivares de tomateiro (Licopersicum esculentum) cultivados em areia lavada. IN: CONGRESSO BRASILEIRO DE CIÊNÇIA DO SOLO, 25., 1995, Viçosa, MG. Resumos expandidos. Viçosa: UFV, 1995. v.3, p. 1339-1341.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 17. | | AZEVEDO NETO, A. D. de; BARRETO, L. P.; BEZERRA NETO, E.; SANTOS, F. V. Efeito da salinidade sobre dois cultivares de tomateiro (Licopersicum esculentum) cultivados em solo. IN: CONGRESSO BRASILEIRO DE CIÊNÇIA DO SOLO, 25., 1995, Viçosa, MG. Resumos expandidos. Viçosa: UFV, 1995. v.3, p. 1342-1344.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
21/02/2017 |
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Data da última atualização: |
21/02/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
BARRETO, L. P.; LUZ, C.; MASCARIN, G. M.; ROBERTS, D. W.; ARRUDA, W.; FERNANDES, E. K. K. |
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Afiliação: |
LUCAS P. BARRETO, UFG; CHRISTIAN LUZ, UFG; GABRIEL MOURA MASCARIN, CNPAF; DONALD W. ROBERTS, UTAH STATE UNIVERSITY; WALQUÍRIA ARRUDA, UFG; EVERTON K. K. FERNANDES, UFG. |
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Título: |
Effect of heat stress and oil formulation on conidial germination of Metarhizium anisopliae s.s. on tick cuticle and artificial medium. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Journal of Invertebrate Pathology, v. 138, p. 94-103, 2016. |
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DOI: |
10.1016/j.jip.2016.06.007 |
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Idioma: |
Inglês |
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Conteúdo: |
The effect of heat stress (45°C) versus non-heat stress (27°C) on germination of Metarhizium anisopliae sensu stricto (s.s.) isolate IP 119 was examined with conidia formulated (suspended) in pure mineral oil or in water (Tween 80, 0.01%), and then applied onto the cuticle of Rhipicephalus sanguineus sensu lato (s.l.) engorged females or onto culture medium (PDAY). In addition, bioassays were performed to investigate the effect of conidia heated while formulated in oil, then applied to blood-engorged adult R. sanguineus females. Conidia suspended in water then exposed to 45°C, in comparison to conidia formulated in mineral oil and exposed to the same temperature, germinated less and more slowly when incubated on either PDAY medium or tick cuticle. Also, conidial germination on tick cuticle was delayed in comparison to germination on artificial culture medium; for example, germination was 13% on tick cuticle 72h after inoculation, in contrast to 61.5% on PDAY medium. Unheated (27°C) conidia suspended in either water or oil and applied to tick cuticle developed appressoria 36h after treatment; whereas only heat-stressed conidia formulated in oil developed appressoria on tick cuticle. In comparison to conidia heated in mineral oil, there was a strong negative effect of heat on germination of conidia heated in water before being applied to arthropod cuticle. Nevertheless, bioassays [based primarily on egg production (quantity) and egg hatchability] exhibited high percentages of tick control regardless of the type of conidial suspension; i.e., water- or oil-formulated conidia, and whether or not conidia were previously exposed to heat. In comparison to aqueous conidial preparations, however, conidia formulated in oil reduced egg hatchability irrespective of heat or no-heat exposure. In conclusion, mineral-oil formulation protected conidia against heat-induced delay of both germination and appressorium production when applied to the cuticle of R. sanguineus. MenosThe effect of heat stress (45°C) versus non-heat stress (27°C) on germination of Metarhizium anisopliae sensu stricto (s.s.) isolate IP 119 was examined with conidia formulated (suspended) in pure mineral oil or in water (Tween 80, 0.01%), and then applied onto the cuticle of Rhipicephalus sanguineus sensu lato (s.l.) engorged females or onto culture medium (PDAY). In addition, bioassays were performed to investigate the effect of conidia heated while formulated in oil, then applied to blood-engorged adult R. sanguineus females. Conidia suspended in water then exposed to 45°C, in comparison to conidia formulated in mineral oil and exposed to the same temperature, germinated less and more slowly when incubated on either PDAY medium or tick cuticle. Also, conidial germination on tick cuticle was delayed in comparison to germination on artificial culture medium; for example, germination was 13% on tick cuticle 72h after inoculation, in contrast to 61.5% on PDAY medium. Unheated (27°C) conidia suspended in either water or oil and applied to tick cuticle developed appressoria 36h after treatment; whereas only heat-stressed conidia formulated in oil developed appressoria on tick cuticle. In comparison to conidia heated in mineral oil, there was a strong negative effect of heat on germination of conidia heated in water before being applied to arthropod cuticle. Nevertheless, bioassays [based primarily on egg production (quantity) and egg hatchability] exhibited high percentages of ... Mostrar Tudo |
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Thesagro: |
Fungo entomogeno; Metarhizium anisopliae. |
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Thesaurus NAL: |
Entomopathogenic fungi; Heat tolerance; Rhipicephalus sanguineus. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
LEADER 02812naa a2200253 a 4500
001 2065020
005 2017-02-21
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1016/j.jip.2016.06.007$2DOI
100 1 $aBARRETO, L. P.
245 $aEffect of heat stress and oil formulation on conidial germination of Metarhizium anisopliae s.s. on tick cuticle and artificial medium.$h[electronic resource]
260 $c2016
520 $aThe effect of heat stress (45°C) versus non-heat stress (27°C) on germination of Metarhizium anisopliae sensu stricto (s.s.) isolate IP 119 was examined with conidia formulated (suspended) in pure mineral oil or in water (Tween 80, 0.01%), and then applied onto the cuticle of Rhipicephalus sanguineus sensu lato (s.l.) engorged females or onto culture medium (PDAY). In addition, bioassays were performed to investigate the effect of conidia heated while formulated in oil, then applied to blood-engorged adult R. sanguineus females. Conidia suspended in water then exposed to 45°C, in comparison to conidia formulated in mineral oil and exposed to the same temperature, germinated less and more slowly when incubated on either PDAY medium or tick cuticle. Also, conidial germination on tick cuticle was delayed in comparison to germination on artificial culture medium; for example, germination was 13% on tick cuticle 72h after inoculation, in contrast to 61.5% on PDAY medium. Unheated (27°C) conidia suspended in either water or oil and applied to tick cuticle developed appressoria 36h after treatment; whereas only heat-stressed conidia formulated in oil developed appressoria on tick cuticle. In comparison to conidia heated in mineral oil, there was a strong negative effect of heat on germination of conidia heated in water before being applied to arthropod cuticle. Nevertheless, bioassays [based primarily on egg production (quantity) and egg hatchability] exhibited high percentages of tick control regardless of the type of conidial suspension; i.e., water- or oil-formulated conidia, and whether or not conidia were previously exposed to heat. In comparison to aqueous conidial preparations, however, conidia formulated in oil reduced egg hatchability irrespective of heat or no-heat exposure. In conclusion, mineral-oil formulation protected conidia against heat-induced delay of both germination and appressorium production when applied to the cuticle of R. sanguineus.
650 $aEntomopathogenic fungi
650 $aHeat tolerance
650 $aRhipicephalus sanguineus
650 $aFungo entomogeno
650 $aMetarhizium anisopliae
700 1 $aLUZ, C.
700 1 $aMASCARIN, G. M.
700 1 $aROBERTS, D. W.
700 1 $aARRUDA, W.
700 1 $aFERNANDES, E. K. K.
773 $tJournal of Invertebrate Pathology$gv. 138, p. 94-103, 2016.
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